Heterogenomic Recombinants from Compatible Nocardiae

Colony mutants of compatible nocardiae displaying variations in recombining capacity.

Colonial morphology mutants of Nocardia erythropolis were isolated following ultraviolet (UV) irradiation. The alleles rou-1/smo-1 were located by recombinant analysis and found to be linked to previously mapped characters. On the basis of recombinant class type patterns obtained from various selective characters it was postulated that the rou-1 allele may span a region of unique nucleotides in...

Characterization of deoxyribonucleic acids from streptomycetes and nocardiae.

The relationships among selected streptomycetes, nocardiae, and mycobacteria have been determined, based upon the base composition of their deoxyribonucleic acid (DNA) and upon the ability of their denatured DNA to anneal with single-stranded reference DNA. The streptomycetes constituted a homogeneous group whose DNA contained between 69 and 73 mole% guanine + cytosine (% GC). Moreover, the str...

Sporulation by some strains of nocardiae and streptomycetes.

Finding Founder Sequences from a Set of Recombinants

Inspired by the current interest in the so–called haplotype blocks we consider a related, complementary problem abstracted from the following scenario. We are given the DNA or SNP sequences of a sample of individuals from a (human) population. The population is assumed to have evolved as an isolate, founded some generations ago by a relatively small number of settlers. Then the sequences in our...

Distilling Artificial Recombinants from Large Sets of Complete mtDNA Genomes

BACKGROUND Large-scale genome sequencing poses enormous problems to the logistics of laboratory work and data handling. When numerous fragments of different genomes are PCR amplified and sequenced in a laboratory, there is a high imminent risk of sample confusion. For genetic markers, such as mitochondrial DNA (mtDNA), which are free of natural recombination, single instances of sample mix-up i...